This product detects novel coronavirus antigens in pseudo-patient nasopharyngeal and oral pharyngeal swabs by cross-current immunoassay.
Widespread application: Applies to hospitals, disease management centers, communities, airports, train stations, customs, schools, businesses, etc.
We recommend aseptic sample collection using polyester sponge samples with PP (polypropylene) sticks.
(1) How to grab the specimens of the mouth and pharynx: The gluteal head is slightly tilted, the mouth is open, and the amygdala of the pharynx on both sides is exposed to the outside. Pass a wipe from the base of the tongue to the base of the tonx and apply to both pharyngeal tonsils. Wipe the posterior wall of the pharynx up and down at least 3 times with a cotton swab.
(2) How to grab the nasopharyngeal sample: Hold the subject's head lightly with one hand, insert a cloth to wipe with one hand into the nostril, and slowly infiltrate the back along the bottom of the inferior nasal passage. The nasal meatus is curved, so don't overdo it to avoid traumatic bleeding. When the upper part reaches the posterior wall of the nasopharynx, gently turn it once (stop the reflex cough) and slowly remove it.
(3) Sample processing: The sample must be treated with the sample buffer provided in this kit as soon as possible (if not processed immediately, store immediately, dry, sterilize, sealed plastic tube), below 2 ° C to 8 ° C. Do not exceed 24 hours — long-term storage at 70 ° C, but avoid freezing and thawing.
Test Method
Bring all reagents to room temperature before testing. The test should be conducted at room temperature.
I. Sample extraction (see Figure 1)
1. Add 400 μL (about 10 drops) of buffer solution vertically to the sample extraction tube, insert the sample into the solution in the sample extraction tube, rotate it about 10 times through the inner wall, and dissolve it in the solution as much as possible.
2. Extrude the tip of the sample along the inner wall of the extraction tube, leaving as much liquid as possible in the tube and taking out the sample.
3. Apply drip.
II. Test Procedure (See Figure 2)
1. Remove the test card from the sticker bag.
2. Add 2 drops (about 80 μL) of the processed sample extract to the sample hole of the test card and activate the timer.
3. Place the test card at room temperature for 15 minutes before reading the results. It could not be read after 20 minutes.
Interpretation of test results
Diagram of test card result judgment:
(1) Invalid result: The test is invalid because there is no reaction line on the quality control line (C line). This experiment is redone
② Negative result: red ribbon, quality control line color (C line)
③ Positive result: Two red bands, the inspection line (t line) and the quality control line (C line), are displayed in color.
